Table 1.
Primers used in the study
| PCR no. and primer | Sequence (5′–3′) | Amplicon size (bp) | Reference |
|---|---|---|---|
| 1 | |||
| cR1 | AAGAATTGAACCAACGCATGA | 7 | |
| insert orf | CTTTGATAAGCCATTCATTC | 326 | This study |
| 2 | |||
| DR2/IR2_F2 | GATTTTCCCAACCGCACC | ||
| DR2/IR2_R2 | ACCTTTATTCGTTACGCCC | 975 | This study |
| 3 | |||
| DR3/IR3_F2 | ATATAGGTGAGAGTATGATAAGTGAG | ||
| ins-orf-M299 | GCTATCAATCTGTCCTCAAA | 325 | This study |
| 4 | |||
| Insert_IS431 | GTAAATGAACTGCAGCTATTG | ||
| IS431F | CTTCTGGTTGGAATGTGTGG | 939 | This study |
| 5 | |||
| IS1272F1 | GCCACTCATAACATATGGAA | 3 | |
| IS1272R1 | CATCCGAGTGAAACCCAAA | 415 | |
| 6 | |||
| DCS F2 | CATCCTATGATAGCTTGGTC | 13 | |
| DCS R1 | CTAAATCATAGCCATGACCG | 342 | |
| 7 | |||
| cR1 | AAGAATTGAACCAACGCATGA | 7 | |
| ins-orf-M299 | GCTATCAATCTGTCCTCAAA | 395 | This study |
| 8 | |||
| cR1 | AAGAATTGAACCAACGCATGA | 7 | |
| DR2/IR2_R2 | ACCTTTATTCGTTACGCCC | 606 | This study |
| 9 | |||
| DR2/IR2_F2 | ACCTTTATTCGTTACGCCC | ||
| ins-orf-M299 | GCTATCAATCTGTCCTCAAA | 764 | This study |