Table 6.
RT-qPCR analysis of the expression of pmrCAB in different strains compared to the level for the laboratory wild-type strain ATCC 17978a
| Strain | Mean fold change ± SD |
||
|---|---|---|---|
| pmrC | pmrA | pmrB | |
| R2 | 224.8 ± 140.6 | 51.7 ± 32.7 | 30.5 ± 18.5 |
| R2 pmrB::Km | 1.43 ± 0.09 | 1.3 ± 0.4 | * |
| R2 pmrB::Km + pmrABWT | 1.0 ± 0.3 | 49.5 ± 8.1 | 189.9 ± 17.0 |
| R2 pmrB::Km + pmrABR2 | 567.0 ± 137.0 | 58.8 ± 6.2 | 77.0 ± 9.2 |
| R5 | 165.2 ± 27.6 | 18.5 ± 0.4 | 10.6 ± 0.3 |
| R5 pmrB::Km | 2.0 ± 1.2 | 0.9 ± 0.2 | * |
| C8 | 292.1 ± 18.3 | 4.3 ± 0.05 | 2.7 ± 0.3 |
| C8 pmrB::Km | 6.8 ± 0.6 | 1.7 ± 0.08 | * |
| C14 | 26.5 ± 3.3 | 4.1 ± 0.7 | 3.2 ± 0.5 |
| C14 pmrB::Km | 16.2 ± 1.8 | 2.9 ± 1.7 | 0.9 ± 0.1 |
a
The values represent three independent samples, with each assessment being repeated in duplicate. *, no expression of pmrB could be detected in these strains.