Table 2.
PFGE analysis of 20 human and 9 companion animal E. coli ST131 isolates compared to a large reference librarya
| Pulsotype(s)b | Present study (n = 29 ST131 isolates) |
Reference library (n = 533 ST131 isolates)c |
|||||||
|---|---|---|---|---|---|---|---|---|---|
| No. (%) of isolates | No. of human isolates (n = 20) | No. of animal isolates (n = 9) | Rank order in library | No. (%) of isolates | No. of human isolates | Distribution |
No. of animal isolates | ||
| Country | No. | ||||||||
| 968 | 12 (41) | 6 | 5 dogs, 1 cat | 1 | 121 (23) | 110 | US | 94 | 11d |
| Canada | 14 | ||||||||
| India | 1 | ||||||||
| Portugal | 1 | ||||||||
| 800 | 4 (14) | 4 | 0 | 2 | 77 (14) | 77 | US | 55 | 0 |
| Canada | 20 | ||||||||
| India | 2 | ||||||||
| 812 | 3 (10) | 3 | 0 | 3 | 29 (5) | 28 | US | 24 | 1d |
| Canada | 1 | ||||||||
| Spain | 2 | ||||||||
| Portugal | 1 | ||||||||
| 905 | 1 (3) | 0 | 1 dog | 4 | 18 (3) | 18 | US | 14 | 0 |
| Canada | 4 | ||||||||
| 807 | 1 (3) | 1 | 0 | 6 | 6 (1) | 6 | US | 4 | 0 |
| Canada | 2 | ||||||||
| 838 | 1 (3) | 1 | 0 | 15 | 3 (0.6) | 3 | US | 3 | 0 |
| 920 | 1 (3) | 0 | 1 dog | 25 | 1 (0.2) | 1 | US | 1 | 0 |
| 915 | 1 (3) | 1 | 0 | 38 | 1 (0.2) | 1 | France | 1 | 0 |
| 975 | 1 (3) | 1 | 0 | 39 | 1 (0.2) | 1 | US | 1 | 0 |
| 796, 976, 1110, and 1153 | 1 (3) each | 3 | 1 doge | NA* | NA* | NA* | NA* | NA | |
a
The reference library currently comprises 533 ST131 isolates from diverse locales, hosts, and clinical contexts (J. R. Johnson, unpublished data).
b
Pulsotypes are defined at the 94% Dice coefficient similarity level and are labeled sequentially as they are encountered.
c
US, United States. NA*, not applicable (these pulsotypes are represented in the reference library only by isolates from the present study); NA, not applicable (no animal isolates in pulsotype).
d
The listed reference collection animal isolates were from the United States.
e
The dog isolate was pulsotype 1153.