Table 4.
Combinatorial sensitivity assay for bromperidol against M. tuberculosis in vitro and ex vivo
| Compound | MIC for M. tuberculosis (mg/liter)a |
|||||
|---|---|---|---|---|---|---|
|
In vitrob |
Fold increased |
Ex vivoc |
Fold increase | |||
| − BPDL | + BPDL | − BPDL | + BPDL | |||
| Bromperidol | 64–128 | ND | 8–16 | ND | ||
| Azithromycin | ND | ND | 8–16 | 1–2 | 8 | |
| Bacitracin | >512 | >512 | 1 | ND | ND | |
| Clarithromycin | 64 | 4 | 8 | 0.5–1 | 0.125 | 4–8 |
| Clofazimine | 0.125–0.25 | 0.015–0.03 | 4–8 | 0.6 | 0.15 | 4 |
| Econazole | 32 | 4–8 | 4–8 | ND | ND | |
| Isoniazid | 0.025 | 0.025 | 1 | ND | ND | |
| Novobiocin | 32–64 | 8–16 | 4 | ND | ND | |
| p-Aminosalicylate | 0.06 | 0.06 | 1 | ND | ND | |
| Rifampin | 0.125 | 0.03 | 4 | 0.125 | 0.03 | 4 |
| Spectinomycin | 80–160 | 2.5 | 64 | 80–160 | 5 | 16–32 |
| Streptomycin | 1 | 0.25 | 4 | ND | ND | |
| Tetracycline | 8 | 8 | 1 | ND | ND | |
| Vancomycin | >256 | >256 | 1 | ND | ND | |
a
MICs were assayed in a range of 2-fold dilutions of antibiotics in the presence or absence of bromperidol (BPDL) at 1/4×MIC. ND, not determined.
b
The MIC was determined in 7H9 liquid medium.
c
The MIC was determined in M. tuberculosis-infected THP1 macrophages.
d
Fold increase in sensitivity in the presence of bromperidol.