Table 2.
MLST, spa types, and DNA microarray hybridization profiles of MRSA isolates M10/0061 and M10/0148 and MSSA isolate 21250_V454
| Gene class and gene(s) | DNA microarraya hybridization profile |
||
|---|---|---|---|
| M10/0061 | M10/0148 | 21250_V454 | |
| Species markers katA, coa, nuc, spa | Pos | Pos | Pos |
| agr group III | Pos | Pos | Pos |
| SCCmec-associated markers | |||
| mecA | Neg | Neg | Neg |
| ΔmecR1 | Neg | Neg | Neg |
| mecR1-mecI | Neg | Neg | Neg |
| ccrA1 | Neg | Neg | Neg |
| ccrB1 | Variable | Variable | Neg |
| Q9XB68-dcs | Neg | Neg | Neg |
| ccrA2-ccrB2 | Neg | Neg | Neg |
| kdp-SCC locus | Neg | Neg | Neg |
| ccrA3 | Variable | Variable | Neg |
| ccrB3 | Variable | Variable | Neg |
| ccrC, ccrA4-ccrB4 | Neg | Neg | Neg |
| Antimicrobial resistance genes | |||
| blaZ | Neg | Neg | Neg |
| blaI-blaR1 | Neg | Neg | Neg |
| erm(A), erm(B) | Neg | Neg | Neg |
| erm(C) | Neg | Neg | Neg |
| lnu(A) | Neg | Neg | Neg |
| msr(A), mph(C) | Neg | Neg | Neg |
| aacA-aphD | Neg | Neg | Neg |
| aadD | Neg | Neg | Neg |
| aphA3-sat | Neg | Neg | Neg |
| dfrS1 | Neg | Neg | Neg |
| far1 | Neg | Neg | Neg |
| mupA | Neg | Neg | Neg |
| tet(K) | Neg | Neg | Neg |
| tet(M) | Neg | Neg | Neg |
| cat | Neg | Neg | Neg |
| cfr | Neg | Neg | Neg |
| fexA | Neg | Neg | Neg |
| fosB | Neg | Neg | Neg |
| tet efflux | Pos | Pos | Pos |
| Virulence-associated genes | |||
| tst1 | Neg | Neg | Neg |
| sea, seb, see, seh | Neg | Neg | Neg |
| sec-sel, sed-sej-ser | Neg | Neg | Neg |
| seg-sei-sem-sen-seo-seu | Neg | Neg | Neg |
| sek-seq | Neg | Neg | Neg |
| lukF-PV–lukS-PV | Neg | Neg | Neg |
| lukF-PV (P83), lukM | Neg | Neg | Pos |
| hla, hld | Pos | Pos | Pos |
| hlb | Pos | Pos | Pos |
| Untruncated hlb | Pos | Pos | Pos |
| sak-chp-scn | Neg | Neg | Neg |
| etA, etB, etD | Neg | Neg | Neg |
| edinA, edinB | Neg | Neg | Neg |
| edinB | Pos | Pos | Pos |
| arcA-arcB-arcC-arcD | Neg | Neg | Neg |
| Capsule type 8 | Pos | Pos | Pos |
The StaphyType Kit (Alere Technologies) was used for DNA microarray analysis. The table shows only the clinically relevant markers, including exotoxins and resistance genes, and does not represent the complete hybridization profile of 334 probes on the array. Full data sets are available upon request. Isolate M10/0061 was CC130/ST130 and had the MLST allelic profile 6-57-45-2-7-58-52, spa type t843, and spa repeat succession 04-82-17-25-17-25-25-16-17. Isolate M10/0148 was CC130/ST1764 and had the MLST allelic profile 6-57-45-2-7-193-52, spa type t373, and spa repeat succession 04-17-25-17-25-16-17. Isolate 21250_V454 was CC130/ST2024 and had the MLST allelic profile 6-57-45-2-219-58-52, spa type t8403, and spa repeat succession 04-82-17-25-25-25-16-17. Pos, positive; Neg, negative; Variable, MRSA isolates M10/0061 and M10/0148 exhibited weak and/or variable signals for ccrB1, ccrA3, and ccrB3 in the primer-directed protocol but yielded positive signals for ccrB1 and ccrB3 in the random protocol outlined in the Materials and Methods.