Fig. 1.
3′-azido-ddG-resistant xxHIV-1LAI was selected by serial passage of virus in increasing concentrations of 3′-azido-ddG. Passaged virus was phenotyped for 3′-azido-ddG susceptibility (EC50, 10.1 ± 2.5 μM at passage 88) and compared to the control wild-type xxHIV-1LAI passaged in parallel without 3′-azido-ddG (EC50, 1.91 ± 1.16 μM at passage 88) to determine resistance at every fifth passage. Standard population sequencing was performed at every 10th passage and identified the indicated RT mutations that differed from the starting virus population.