Table 1.
Resistance and cross-resistance of wild-type (WT) and 3′-azido-ddG-selected virus
| Drug | EC50 (μM)a |
Fold resistanced | P valuee | |
|---|---|---|---|---|
| WTb | Selected virusc | |||
| ddI | 4.3 ± 0.2 | 26.6 ± 1.2 | 6.2 | <0.0001 |
| 3′-azido-ddG | 1.9 ± 1.2 | 10.1 ± 2.5 | 5.3 | <0.001 |
| 3TC | 2.2 ± 1.8 | 10.5 ± 4.1 | 4.8 | <0.05 |
| ABC | 8.9 ± 0.5 | 37.2 ± 6.3 | 4.2 | <0.05 |
| TFV | 2.1 ± 0.7 | 5.4 ± 0.7 | 2.5 | <0.05 |
| D4T | 14.1 ± 3.0 | 23.2 ± 1.1 | 1.5 | <0.01 |
| 3′-azido-ddA | 3.7 ± 0.6 | 5.6 ± 0.2 | 1.5 | <0.05 |
| AZT | 0.3 ± 0.2 | 0.4 ± 0.3 | 1.3 | NS |
a
Means ± standard deviations of three experiments.
b
WT virus passaged in parallel with the 3′-azido-ddG selection virus in the absence of drug; genotype was confirmed by sequencing.
c
Virus isolated from passage 88 of the 3′-azido-ddG selection that contained L74V, F77L, L214F, K476N, and V518I by standard population sequencing.
d
Fold resistance compared with wild-type xxHIV-1LAI.
e
Statistical significance compared to wild-type xxHIV-1LAI, determined by a two-sample Student's t test. NS, not significant.