Table 4.
3′-Azido-ddG susceptibility of recombinant HIV-1 with RT derived from single-genome amplifications
| Clonea | Genotypeb | EC50 (μM)c | Fold resistanced | P valuee |
|---|---|---|---|---|
| 1 | L74V/F77L/V106I/E122K/L214F/R277K/S447N/V518I/V531I | 4.5 ± 1.2 | 3.2 | <0.05 |
| 2 | L74V/F77L/V106I/E122K/L214F/K476N | 4.8 ± 1.0 | 3.4 | <0.01 |
| 3 | L74V/F77L/V106I/L214F/R277K/K476N | 5.3 ± 1.8 | 3.8 | <0.05 |
| 6 | L74V/F77L/V106I/E122K/L214F/S447N/P510T/L533M | 5.0 ± 1.6 | 3.6 | <0.05 |
| 7 | L74V/F77L/E122K/L214F/K476N | 4.7 ± 0.7 | 3.4 | <0.01 |
| 10f | L74V/F77L/L214F/K476N | 5.6 ± 1.5 | 4.0 | <0.01 |
| 13 | L74V/F77L/L214F/R277K/V435I/P510T/V518I | 5.4 ± 0.9 | 3.8 | <0.01 |
| Bulk | Mixture | 2.31 ± 0.01 | 2.2 | <0.05 |
Recombinant clones were produced by amplifying full-length RT from single genomes or as a population (bulk) and cloning into the xxHIVLAI-3D vector for infectious virus production by electroporation into MT-2 cells.
Genotypes identified by single-genome sequencing (Table 3) that are not listed were cloned but determined to be growth defective.
Means ± standard deviations of three experiments.
Fold resistance was determined compared to the xxHIV-1LAI clone (EC50, 1.40 ± 0.05 μM).
Statistical significance compared to wild-type xxHIV-1LAI-MO, determined by using a two-sample Student's t test.
Clone 10 contains a silent G-to-A transition at nucleotide 423 that encodes G141 in the HIV-1xxLAI background.