Fig. 3.

Effects of gerBC mutations on germination of spores with the FB10 or FB8 background. (A) Germination of spores with the FB10 background in the presence of l-asparagine (left) or l-valine (right). The percentages of DPA release were normalized as described in Materials and Methods, and the maximum number of RFU obtained for each l-valine-mediated germination reaction was considered to be 100%. The germination curve of wild-type spores in the absence of germinant is shown and used as the negative control. Data represent means ± SD for at least three independent measurements. (B) Germination of spores with the FB8 background in the presence of l-asparagine (left) or l-valine (right). The percentages of DPA release were normalized as described above. The germination curve of spores with wild-type GerBC in the absence of germinants is shown. Data represent means ± SD for at least three independent measurements. (C) Initial rates of germination of spores with the FB10 background. Rates were calculated as described in the legend for Fig. 1D. (D) Initial rates of germination of spores with the FB8 background.