Table 1.
Kinetic analysis of germination of wild-type and mutant gerBC spores with l-valine, AGFK, or l-asparagine alonea
| gerBC strain | Kinetics of germination with: |
|||
|---|---|---|---|---|
|
l-Asparagineb |
l-Valine |
|||
| C50 (mM)c | Vmax (min−1) | C50 (mM) | Vmax (min−1) | |
| PS832 | ||||
| WT | 1.3 ± 0.4 | 117 ± 9 | 1.6 ± 0.2 | 113 ± 5 |
| BC1 | NAd | 0 | 2.8 ± 0.5 | 120 ± 7 |
| BC2 | 1.7 ± 0.6 | 98 ± 11 | 2.1 ± 0.2 | 115 ± 3 |
| BC3 | NA | 0 | 3.3 ± 0.4 | 121 ± 6 |
| BC4 | NA | 0 | 3.1 ± 0.5 | 125 ± 6 |
| BC5 | 0.7 ± 0.1 | 105 ± 3 | 4.2 ± 0.4 | 126 ± 4 |
| BC6 | 2.0 ± 0.5 | 104 ± 7 | 2.3 ± 0.2 | 114 ± 4 |
| BC7 | 1.1 ± 0.1 | 104 ± 2 | 5.6 ± 0.7 | 135 ± 7 |
| BC8 | NA | ∼0 | 1.5 ± 0.4 | 113 ± 8 |
| BC9 | NA | ∼0 | 2.1 ± 0.3 | 114 ± 4 |
| BC10 | NA | ∼0 | 2.7 ± 0.5 | 122 ± 8 |
| FB10 | ||||
| WT | 0.5 ± 0.04 | 107 ± 2 | 1.7 ± 0.1 | 111 ± 2 |
| BC4 | NA | 0 | 2.4 ± 0.3 | 118 ± 4 |
| BC5 | 0.3 ± 0.1 | 109 ± 3 | 1.7 ± 0.2 | 111 ± 4 |
| BC6 | NA | 0 | 4.0 ± 0.4 | 124 ± 5 |
| BC8 | NA | 0 | 4.8 ± 0.6 | 131 ± 7 |
| BC9 | NA | 0 | 2.5 ± 0.2 | 118 ± 3 |
| BC10 | NA | 0 | 2.6 ± 0.3 | 116 ± 4 |
a
Kinetic parameters of spore germination were determined as described in Materials and Methods and the legends to Fig. 2 and 3.
b
GFK at 10 mM each were present in the l-asparagine germination of spores with the PS832 (wild-type) background but not in the germination of spores with the FB10 background.
c
C50, germinant concentration that stimulates the half-maximal initial rate of germination.
d
NA, not applicable, since the germination rate was zero for these spores.