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. 2011 Aug;193(16):4134–4142. doi: 10.1128/JB.00288-11

Fig. 1.

Fig. 1.

(A) Evidence of chromosomal deletion of cls1 and/or cls2 in S. aureus cls1, cls2, and cls1 cls2 mutants by PCR. Primer pairs annealing upstream and downstream of the cls1 (TK161/164; upper line) and cls2 (TK149/152; lower line) structural genes were used to confirm cls1 and cls2 chromosomal deletions. The source of genomic DNA used as a template is depicted above each PCR product. wt, wild type; cls1, S. aureus cls1; cls2, S. aureus cls2; cls1/2, S. aureus cls1 cls2. (B) Amino acid sequence alignment of S. aureus Cls1 and Cls2 using CLUSTAL W. Asterisks denote sites of amino acid identity; colons, sites of amino acid similarity. Boxed regions correspond to predicted transmembrane helices (TMH-1 and TMH-2), using HMMTOP 2.0 and TopPredII secondary structure prediction software, and conserved domains of the active site [HxK(x)4D(x)6GSxN, shaded amino acids] of the phospholipase D (PLD) family, identified by NCBI Blastp.