Table 2.
Plasmids used in this study
| Plasmid | Description | Restriction sites | Source or reference |
|---|---|---|---|
| pDB81 | pUC19/pC194 E. coli and S. aureus shuttle vector | D. J. Bartels | |
| pKORI | Original plasmid knockout vector | AttB/AttP | 1 |
| pKORcls1 | cls1 knockout vector | This study | |
| pKORcls2 | cls2 knockout vector | This study | |
| pTKOcls1 | pDB81 with cls1 (SAOUHSC_1310) including 460 bp upstream of translation start codon | KpnI/SacI | This study |
| pTKOcls2 | pDB81 with cls2 (SAOUHSC_2323) including 738 bp upstream of translation start codon | KpnI/SacI | This study |
| pRB474 | E. coli/S. aureus shuttle vector | 35 | |
| pRB474cls1 | pRB473 with cls1 (SAV1317) | BamHI/BamHI | This study |
| pRB474cls2 | pRB473 with cls2 (SAV2088) | BamHI/SphI | This study |
| pCM29 | sGFP expression under constitutive sarAP1 promoter | 33 |