Fig. 1.

Growth phenotype and autoradiogram of the products of [¹⁴C]palmitate labeling of strains L43 and LSC9. (A) Strains JH642, L43, and LSC9 were grown as described in Materials and Methods. Cells were harvested, washed, and resuspended in fresh MM. Strain L43 was supplemented (○) or not supplemented (□) with 0.01% MIV, while strains JH642 (▿) and LSC9 (▴) were grown in MM-MIV. Strain LSC9 was grown without IPTG. (B) UFAs synthesized by strains JH642, L43, and LSC9 at 25°C. Cultures were grown to the mid-exponential phase at 37°C in minimal medium. Two milliliters of the cultures was challenged with 0.2 μCi [¹⁴C]palmitate and further shifted to 25°C for 2 h. The lipids were then extracted and transesterified, and the resulting methyl esters were separated by thin-layer chromatography silver-nitrate-impregnated silica plates. Lanes 1 and 3, strain JH642; lane 2, strain L43; lane 4, strain LSC9.