Fig. 2.

Growth phenotypes and autoradiogram of the products of [¹⁴C]palmitate labeling of LSC28. (A) Strains JH642 (▴) and LSC28 (⋄) were grown as described in Materials and Methods. Cells were harvested, washed, and resuspended in fresh medium supplemented with 0.01% MIV. After ∼15 h of growth, 0.5 mM IPTG was added to a culture of strain LSC28 (dashed arrow). (B) Argentation-thin-layer chromatography analysis of fatty acid methyl esters. Cells were grown at 37°C as described as in A. At the growth stages indicated by the solid arrows in A, 2 ml of the cultures was challenged with 0.2 μCi [¹⁴C]palmitate and further shifted to 25°C for 2 h. The lipids were then extracted and transesterified, and the resulting methyl esters were separated by thin-layer chromatography silver-nitrate-impregnated silica plates. Lane 1, JH642; lanes 2 to 5, LC28.