Fig. 4.

Survival assays. (A) The 11168H wild-type, Cj1556 mutant, and Cj1556 complement strains (Cj1556 comp) were cocultured with Caco-2 intestinal epithelial cells for 24 h, followed by assessment of the number of bacteria in the coculture medium. (B to E) Further survival assays were performed, in which the C. jejuni strains were grown under microaerobic (B and C) and aerobic (D and E) conditions in brucella broth (B and D) or tissue culture medium (C and E). Then, the numbers of viable bacteria were assessed. The asterisks denote a statistically significant difference (P < 0.05) for the Cj1556 mutant compared to the 11168H wild-type strain.