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Electrophoretic mobility shift assays indicate that Cj1556 binds to a DNA promoter sequence upstream of the Cj1556 gene. Shown is a native Western blot for recombinant 6×His-tagged Cj1556 protein hybridized to DNA fragments representing the upstream promoter sequences of Cj1556 and flaA and flgK (both negative controls) following separation on a Tris-glycine gel under nondenaturing conditions.
