Table 1.
Bacterial strains, plasmids, and primers
| Strain, plasmid, or primer | Genotype, description,a or sequenceb | Parent, reference, or construction |
|---|---|---|
| Strains | ||
| BB7 | Vibrio harveyi | 55 |
| BB22 | V. parahaemolyticus | 5 |
| LM1017 | luxO1017 flgB313L::lux (luxO* constitutive allele) (TR) | 41 |
| LM4476 | luxO1017 (TR) | LM1017 × pLM1776 (allelic replacement) |
| LM5312 | “Wild type” (OP) | BB22 |
| LM5431 | opaR2 (TR) | 59 |
| LM5392 | flaM1P::EzKan opaR14 (TR) | LM5314 |
| LM5674 | ΔopaR1 (TR) | BB22c |
| LM5738 | flgB1L::Tn5lux(Kanr) opaR2 (TR) | LM5431d |
| LM5949 | ΔopaR1flgCL3061::lacZ (Camr) (TR) | LM5674e |
| LM6633 | “Wild type” (OP) | LM5674 × pLM1950 (allelic replacement) |
| LM6693 | flgB1L::lux(Kanr) (OP) | LM5738 × pLM1950 (allelic replacement) |
| LM6820 | ΔopaR1 ΔswrT2::lacZ (Camr) (TR) | LM5674 |
| LM7595 | LM6693/pLM3286 | |
| LM7919 | BB7/pLM1877 | |
| LM7920 | BB7/pLM3286 | |
| LM7921 | BB7/pLM3942 | |
| LM7956 | LM6693/pLM1877 | |
| LM8602 | ΔopaR1flgCL3061::lacZ (Camr) scrC292::Tn5lux(Kanr) (TR) | 15 |
| LM9513 | LM5674/pLM3286 | |
| LM9514 | LM5674/pLM1877 | |
| LM9515 | LM4476/pLM3286 | |
| LM9516 | LM4476/pLM1877 | |
| LM9651 | LM6633/pLM3942 | |
| LM9652 | LM6633/pLM3286 | |
| LM9653 | LM6633/pLM1877 | |
| LM9655 | LM6693/pLM3942 | |
| LM9656 | LM5674/pLM3942 | |
| LM9688 | ΔluxO::Genr (OP) | LM4476 × pLM3294 (allelic replacement) |
| LM9736 | flgB313L::lux luxO1017opaR3282::TnlacZ/in (Camr) (TR) | LM1017 × pLM3282 (allelic replacement) |
| LM9738 | opaR3282::TnlacZ/in (TR) | LM5312 × pLM3282 (allelic replacement) |
| LM9752 | luxO1017 opaR3282::TnlacZ/in (TR) | LM4476 × pLM3282 (allelic replacement) |
| LM9757 | flgB313L::lux ΔluxO::Genr (OP) | LM1017 × pLM3294 (allelic replacement) |
| LM9847 | ΔluxO::Genr (OP) | LM5312 × pLM3294 (allelic replacement) |
| LM9849 | ΔopaR1 ΔluxO::Genr (TR) | LM5674 × pLM3294 (allelic replacement) |
| LM9903 | ΔluxO::GenropaR3282::TnlacZ/in (TR) | LM9688 × pLM3282 (allelic replacement) |
| LM9946 | flgCL3061::lacZ (Camr) scrC292::Tn5lux(Kanr) (OP) | LM8602 × pLM1950 (allelic replacement) |
| LM10036 | ΔopaR1 VPA1435::Tn5lux(Kanr) ΔswrT2::lacZ (Camr) (TR) | LM6820f |
| LM10037 | VPA1435::Tn5lux(Kanr) ΔswrT2::lacZ (Camr) (OP) | LM10036 × pLM1950 (allelic replacement) |
| Plasmids | ||
| pLM1776 | TetrflgBL+ cosmid | 59 |
| pLM1877 | Genr Apr IPTG-inducible expression vector | 8 |
| pLM1950 | Tetr cosmid containing opaR locus from LM5312 (opaR+) | 40 |
| pLM3230 | Tetr cosmid containing 4-kb luxO locus from LM5674 (luxO+) | pLAFR3 |
| pLM3282 | opaR3282::TnlacZ/in (Camr) | pLM1950 |
| pLM3286 | Genr Apr IPTG-inducible luxOU operon from LM5674 (luxO+) | pLM1877 |
| pLM3294 | ΔluxO::Genr | pLM3230 |
| pLM3942 | Genr Apr IPTG-inducible luxOU operon from LM1017 (luxO*) | pLM1877 |
| Primers | ||
| Jkluxofbam | CTTGGATCCGCAAAGCGTAATGCGATTATTG | |
| Jkluxorpst | CTGCTGCAGCACTAAGTAGCCACTTGAGACTGC | |
| Δluxogen1 | GATCGACATTAATATTGTCGGTACAGGTAGAGATGCCATTGAAAGTCTCAATCATCGTGTAGGCTGGAGCTGCTTC | |
| Δluxogen2 | CATTCCATGCTTGTAGTTTGCGGTAAATCGTTGACGGACTGACATCAAGATACCCAGCGCATATGAATATCCTCCTTAG | |
| VP1386for | CCAGTCAATGGTGCAAAAATTGAGG | |
| VP1386rev | CGAAACTGTACATCAGCTAAATGC | |
| VPA1026for | GGCATTGTCTCTGCTGCAAAGCTCG | |
| VPA1026rev | GCCAAGACATATTCTTTTCCTACTCGC | |
| VP2232for | CTGTGTTGGTTAAGCGTATTGCTCAC | |
| VP2232rev | CGTTGCATCGCTTGGCTTAAGTATTTG |