Table 1.
Bacterial strains, plasmids, and oligonucleotides
| Strain, plasmid, or oligonucleotide | Genotype or sequence | Source or referencea |
|---|---|---|
| Strains | ||
| CAG18500 | MG1655 thi-39::Tn10 | 45 |
| GOB125 | Hfr P4X ΔrimM102 yfiB::nptI rpoB2063 (sdr-47) rsd2423::miniTn10Cm | |
| GOB162 | Hfr P4X rbfA::Kmr | 7 |
| GOB249 | Hfr P4X rsd2423::miniTn10Cm | |
| GOB250 | Hfr P4X rpoB2063 (sdr-47) rsd2423::miniTn10Cm | |
| GOB307 | Hfr P4X ΔrimM102 yfiB::nptI rpoC2064 (sdr-36) rsd2423::miniTn10Cm | |
| GOB434 | Hfr P4X lacI′-TrplS-PmetY-metY-metY-T1T2-lacZ | 5 |
| GOB435 | Hfr P4X lacI′-TrplS-PmetY-metY-lacZ | 5 |
| GOB750 | GOB434 nusA94 argG2424::miniTn10Cm | 5 |
| GOB752 | GOB435 nusA94 argG2424::miniTn10Cm | 5 |
| GOB838 | Hfr P4X lacI′-rplSt-PmetY-metY-TrplS-lacZ | 5 |
| GOB868 | GOB838 nusA94 arg2424::miniTn10Cm | 5 |
| GOB896 | GOB434 rpoB2063 (sdr-46) thi-39::Tn10 | |
| GOB898 | GOB434 rpoB+thi-39::Tn10 | |
| GOB900 | GOB434 rpoC2064 (sdr-36) thi-39::Tn10 | |
| GOB902 | GOB434 rpoC+thi-39::Tn10 | |
| GOB904 | GOB435 rpoB2063 (sdr-46) thi-39::Tn10 | |
| GOB906 | GOB435 rpoB+thi-39::Tn10 | |
| GOB906 | GOB435 rpoB+thi-39::Tn10 | |
| GOB908 | GOB435 rpoC2064 (sdr-36) thi-39::Tn10 | |
| GOB910 | GOB435 rpoC+thi-39::Tn10 | |
| MW37 | Hfr P4X ΔrimM102 yfiB::nptI | 33 |
| MW100 | Hfr P4X | 51 |
| MW234 | Hfr P4X rpoB2063 (sdr-46) thi-39::Tn10 | |
| MW237 | Hfr P4X rpoC2064 (sdr-36) thi-39::Tn10 | |
| MW171 | Hfr P4X ΔrimM102 yfiB::nptI rsd2423::miniTn10Cm | |
| MW172 | Hfr P4X ΔrimM102 yfiB::nptI rpoB2063 (sdr-46) rsd2423::miniTn10Cm | |
| MW176 | Hfr P4X rsd2423::miniTn10Cm | |
| MW177 | Hfr P4X rpoC2064 (sdr-36) rsd2423::miniTn10Cm | |
| MW181 | Hfr P4X rpoB2063 (sdr-46) rsd2423::miniTn10Cm | |
| MW286 | GOB838 rpoB2063 (sdr-46) thi-39::Tn10 | |
| MW288 | GOB838 rpoB+thi-39::Tn10 | |
| MW289 | GOB838 rpoC2064 (sdr-36) thi-39::Tn10 | |
| PW102 | Hfr P4X ΔrimM102 yfiB::nptI sdr-36 = rpoC2064 (Dupl. of codons 115 to 116; AGTTAT) | 7 |
| PW112 | Hfr P4X ΔrimM102 yfiB::nptI sdr-46 = rpoB2063 (ATC to AGC in codon 905) | 7 |
| PW113 | Hfr P4X ΔrimM102 yfiB::nptI sdr-47 = rpoB2063 (ATC to AGC in codon 905) | 7 |
| PW157 | Hfr P4X ΔrimM102 yfiB::nptI zhc-2421::Tn10 | G. O. Bylund et al.b |
| Plasmids | ||
| pCW2 | Strr Spcr′rpoC yjaZ thiCEFSGH rsd2423::miniTn10Cm nudC hemE nfi yjaG hupA yjaH zraP zraS′ | |
| pJML007 | bla cat′ araC PBAD-nusA | 5 |
| pMW498 | bla cat′ araC PBAD-His6-nusA | |
| Oligonucleotides | ||
| nusA-BamHI-F | 5′-TTTTGGATCCAACAAAGAAATTTTGGCTGTAG-3′ | |
| nusA-HindIII-R | 5′-TTTTAAGCTTTATTACGCTTCGTCACCG-3′ |
a
Unless otherwise noted, the origin was this study.
b
G. O. Bylund, O. P. Persson, J. M. Lövgren, and P. W. Wikström, unpublished data.