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Dithionite-reduced FrcB is oxidized in the presence of FeCl3. FrcB (2 μM) reduced with dithionite as described in the text was treated with 500 μM FeCl3 or air-saturated buffer, and the absorption spectrum of each was subtracted from the reduced spectrum. Peaks at 428 and 561 nm are noted. The tick marks on the y axis represent a change in absorption of 0.05.
