Fig. 4.

Involvement of the GlxR-binding sites in regulation of in vivo expression of the target genes. The β-galactosidase activity of C. glutamicum strains chromosomally integrated with the target promoter fused with the lacZ reporter gene was measured. Activities derived from the native (white bars) or GlxR-binding site-mutated (black bars) promoters at the stationary phase of growth in nutrient-rich A medium with 1% glucose (Glc) or acetate (Ace) are shown. The activity is the mean value from at least three independent cultivations. Error bars indicate standard deviations. ND, not detected.