Fig. 5.

GlxR attenuates upregulation of citH (cgR_0087) expression by CitAB in the presence of citrate. The β-galactosidase activities of C. glutamicum strains chromosomally integrated with the citH promoter fused with the lacZ reporter gene were measured. Activities derived from the native (white bars) or GlxR-binding site-mutated (black bars) promoters at the exponential phase of growth in nutrient-rich A medium with 1% glucose (Glc), citrate (Cit), or glucose-citrate (Glc+Cit) are shown. The activity is the mean value from at least three independent cultivations. Error bars indicate standard deviations.