Strains |
|
|
E. coli
|
|
|
JM109 |
recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ (traD36 proAB+lacIqlacZΔM15) dam dcm supE44 hsdR17 thi leu rpsL
|
Takara |
JM110 |
lacY galK galT ara tonA thr tsx Δ(lac-proAB)/F′ (traD36 proAB+lacIqlacZΔM15) |
67 |
BL21(DE3) |
F−ompTgaldcmlonhsdSB (rB− mB−) λ(DE3) |
74 |
C. glutamicum
|
|
|
R |
Wild-type strain |
88 |
KT7 |
R with deletion in glxR
|
79 |
KT23 |
R with Strep-tag II-tagged glxR
|
This study |
ΔcyaB mutant |
R with deletion in cyaB
|
57 |
KT25 |
ΔcyaB mutant with Strep-tag II-tagged glxR
|
This study |
Plasmids |
|
|
pCold |
Apr; cold-inducible expression |
Takara |
pGEM T-Easy |
Vector |
Promega |
pCRA725 |
Kmr; suicide vector containing the B. subtilissacB gene |
36 |
pCRA741 |
Kmr; pCRA725 with a 2.0-kb PCR fragment from strain-specific island 7 and a 3.1-kb PCR fragment containing the E. colilacZ gene |
38 |
pCRC619 |
Kmr; pCRA725 with a 2.4-kb fragment containing a Strep-tag II-tagged glxR gene |
This study |
pCRC620 |
Apr: pColdI with a 684-bp fragment containing the glxR gene |
This study |