Table 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristics | Source or reference |
|---|---|---|
| Strains | ||
| E. coli | ||
| JM109 | recA1 endA1 gyrA96 thi hsdR17 supE44 relA1 Δ(lac-proAB)/F′ (traD36 proAB+lacIqlacZΔM15) dam dcm supE44 hsdR17 thi leu rpsL | Takara |
| JM110 | lacY galK galT ara tonA thr tsx Δ(lac-proAB)/F′ (traD36 proAB+lacIqlacZΔM15) | 67 |
| BL21(DE3) | F−ompTgaldcmlonhsdSB (rB− mB−) λ(DE3) | 74 |
| C. glutamicum | ||
| R | Wild-type strain | 88 |
| KT7 | R with deletion in glxR | 79 |
| KT23 | R with Strep-tag II-tagged glxR | This study |
| ΔcyaB mutant | R with deletion in cyaB | 57 |
| KT25 | ΔcyaB mutant with Strep-tag II-tagged glxR | This study |
| Plasmids | ||
| pCold | Apr; cold-inducible expression | Takara |
| pGEM T-Easy | Vector | Promega |
| pCRA725 | Kmr; suicide vector containing the B. subtilissacB gene | 36 |
| pCRA741 | Kmr; pCRA725 with a 2.0-kb PCR fragment from strain-specific island 7 and a 3.1-kb PCR fragment containing the E. colilacZ gene | 38 |
| pCRC619 | Kmr; pCRA725 with a 2.4-kb fragment containing a Strep-tag II-tagged glxR gene | This study |
| pCRC620 | Apr: pColdI with a 684-bp fragment containing the glxR gene | This study |