Table 1.

Details of Austrian and Dutch BSE cases until March 2011^a

Case no.	Yr of birth	Age (mo)	Screening testb	Confirmation testc	Status at death	Typing resultd
AU01	1996	70	PCW	OIE-WB	Healthy	C (A1B2)
AU02	1994	134	TeSeE	OIE-WB	Healthy	C (A1B2)
AU03	1992	155	LIA	IHC	Healthy	C (A1B2)
AU04	2000	70	LIA	IHC	Healthy	C (A1B2)
AU05	1993	149	LIA	OIE-WB	Fallen st.	CP4 (A2B2)
AU06	1996	130	LIA	IHC	Fallen st.	L (A4B1)
AU07	1997	150	PCP	Bio-Rad-WB	Healthy	L (A4B1)
AU08	1995	180	PCP	Bio-Rad-WB	Fallen st.	H (A2B4)
NL73	1996	91	PCW	IHC	Healthy	C (A1B2)
NL74	1996	90	PCW	IHC	Healthy	C (A1B2)
NL86	1999	140	PS	IHC	Healthy	C (A1B2)
NL87	1997	162	PCW	IHC	Fallen st.	C (A1B2)
NL88	1996	174	PCW	IHC	Fallen st.	L (A4B1)

^aAll Austrian cases were investigated for the type of BSE for the first time, as well as the most recent Dutch cases (NL86 to -88), plus some confirmed C BSE cases from a previous study (NL73, NL74) in which all Dutch cases had been analyzed (15). Fallen st., fallen stock (i.e., animals that died on the farm).

^bPCW = Prionics-Check Western blot; TeSeE = enzyme-linked immunosorbent assay (ELISA)-like Bio-Rad TeSeE test; LIA = ELISA-like Prionics-Check luminescence immunoassay; PCP = immunochromatographic lateral diffusion Prionics-Check PrioSTRIP test; PS = Roche PrionScreen ELISA-like test.

^cOIE-WB = World Organisation for Animal Health (OIE)-recognized methods using scrapie-associated fibril (SAF) purification and Western blot detection; IHC = OIE-recognized method using immunohistochemistry; Bio-Rad-WB = an EU-recognized PrP^res preparation method, followed by Western blotting.

^dTyping results (and classifications, shown in parentheses) were derived from Fig. 1. AU05 is a C-type BSE case with intermediately high MAb 12B2 reactivity and unusually high MAb P4 binding comparable to that of 12B2. Such high P4 affinity has not been observed previously, neither for intact bovine PrP nor its fragments (Fig. 2).