Table 2.
Distribution of mutations in QRDRs of the gyrA gene in M. tuberculosis resistant to FQ strainsa
| Sequencing result | Molecular assay result |
|
|---|---|---|
| Pyrosequencing | MTBDRsl | |
| G88C (88 GGC-GCC), n = 1 | G88C (88 GGC-GCC), n = 1 | Unreadable |
| A90V (90 GCG-GTG), n = 4 | A90V (90 GCG-GTG), n = 4 | Δwt2 + MUT1 (codon 90), n = 2; Δwt2 (codon 90), n = 1; unreadable n = 1 |
| S91P (91 TCG-CCG), n = 1 | S91P (91 TCG-CCG), n = 1 | Δwt2 + MUT2 (codon 91), n = 1 |
| D94G (94 GAC-GGC), n = 13 | D94G (94 GAC-GGC), n = 13 | Δwt3 + MUT3C (codon 94), n = 12; Δwt3 + MUT3C + MUT3A (codon 94), n = 1 |
| D94A (94 GAC-GCC), n = 2 | D94A (94 GAC-GCC), n = 2 | Δwt3 + MUT3A (codon 94), n = 1; unreadable, n = 1 |
| D94N (94 GAC-AAC), n = 3 | D94N (94 GAC-AAC), n = 3 | Δwt3 + MUT3B (codon 94), n = 3 |
| D94Y (94 GAC- TAC), n = 1 | D94Y (94 GAC-TAC), n = 1 | Δwt3 + MUT3B (codon 94), n = 1 |
| Wild type, n = 3 | Wild type, n = 3 | Wild type, n = 3 |
| Mixed 90 GCG/GTG + 94 GAC/GGC, n = 1 | Mixed | wt + MUT1 + MUT3C, n = 1 |
| Mixed 94 GAC/AAC/GCC, n = 2 | Mixed | Δwt3 + MUT3A + MUT3B, n = 1; wt + MUT3A + MUT3B + MUT3C, n = 1 |
a
All results are presented as the amino acid change(s), followed by the nucleotide change(s) in parentheses, in gyrA. n, Number of mutations.