Table 2.
Control isolates used in assay optimization
| ID | Strain | Resistance gene(s)a | Identified target(s)b | Source or referencec |
|---|---|---|---|---|
| JIE 251 | E. coli | blaCTX-M-3 | CTX-M-1 group | 17 |
| JIE 059 | E. coli | blaCTX-M-9 | CTX-M-9 group | 17 |
| JIE 088 | E. coli | blaCTX-M-14 | CTX-M-9 group | 17 |
| JIE 236 | E. coli | blaCTX-M-15 | CTX-M-1 group | 17 |
| JIE 298 | E. coli | blaCTX-M-24 | CTX-M-9 group | 17 |
| JIE 058 | E. coli | blaCTX-M-27 | CTX-M-9 group | 17 |
| JIE 137 | K. pneumoniae | blaCTX-M-62 | CTX-M-1 group | 17 |
| PA 185 | P. aeruginosa | blaSHV-5 | SHV-5/12 | 17 |
| JIE 008 | K. pneumoniae | blaSHV-12 | SHV-5/12 | 17 |
| JIE 273 | P. mirabilis | blaVEB-6 | VEB | 16 |
| JIE 084 | E. coli | blaCTX-M-9, blaCTX-M-14 | CTX-M-9 group | 17 |
| JIE162 | K. pneumoniae | blaCTX-M-15, blaSHV-12 | CTX-M-1; SHV-5/12 | 17 |
| JIE1385 | E. coli | blaCTX-M-14, blaCMY-2 | CTX-M-9 group | 17 |
| ATCC 25922 | E. coli | − | ATCC | |
| ATCC 13883 | K. pneumoniae | − | ATCC | |
| ATCC 12453 | P. mirabilis | − | ATCC | |
| ATCC 13047 | E. cloacae | − | ATCC | |
| ATCC 700603 | K. pneumoniae | blaSHV-18 | − | ATCC |
| JIE142 | K. pneumoniae | blaSHV-11 | − | Thomas and Olma |
| JIE181 | K. pneumoniae | blaSHV-28 | − | Thomas and Olma |
| JIE203 | K. pneumoniae | blaSHV-109 | − | Thomas and Olma |
| N6994 | P. aeruginosa | blaIMP-1 | − | Bell and Turnidge |
| WCH 1824 | K. pneumoniae | blaIMP-4 | − | Bell and Turnidge |
| JIP 144 | P. aeruginosa | blaIMP-7 | − | Bell and Turnidge |
| N12281 | E. cloacae | blaIMP-8 | − | Bell and Turnidge |
| NS249 | S. marcescens | blaIMP-11 | − | Bell and Turnidge |
| 08-26640 | P. aeruginosa | blaVIM-1 | − | Bell and Turnidge |
| 08-037308 | P. aeruginosa | blaVIM-2 | − | Bell and Turnidge |
| N12636 | P. aeruginosa | blaVIM-3 | − | Bell and Turnidge |
| RMH078 | P. aeruginosa | blaVIM-4 | − | Peleg and Wiese; 11 |
| JIP152 | P. aeruginosa | blaSPM-1 | − | Thomas and Olma |
| N15348 | A. baumannii | blaSIM-1 | − | Bell and Turnidge |
| WCH 2677 | P. aeruginosa | blaAIM-1 | − | Bell and Turnidge |
| 09K280459L | E. coli | blaNDM-1 | − | Taylor; 14 |
| KPN2303 | K. pneumoniae | blaKPC-2 | − | Quinn; 15 |
| JIE602 | E. coli | blaCMY-2 | − | Thomas and Olma |
| JIE203 | K. pneumoniae | blaDHA-1 | − | Thomas and Olma |
| J53 | E. coli | blaFOX | − | Ingram |
| 08-251-2244 | K. pneumoniae | blaACT/MIR-1 | − | Thomas and Olma |
| AHC01 | A. hydrophila | blaMOX/CMY-1 | − | Thomas and Olma |
| JIE094 | H. alvei | blaACC-1 | − | Thomas and Olma |
a
All listed genes in control strains were sequenced previously or as part of this study.
b
−, negative by PCR (negative controls).
c
ATCC, American Type Culture Collection; Bell and Turnidge, Jan Bell and John Turnidge, Women's and Children's Hospital, Adelaide, Australia; Taylor, Peter Taylor, Prince of Wales Hospital, Sydney, Australia; Thomas and Olma, Lee Thomas and Tom Olma, Westmead Hospital, Sydney, Australia; Peleg and Wiese, Anton Peleg and Peter Wiese, Royal Melbourne Hospital, Melbourne, Australia; Ingram, Paul Ingram, Sir Charles Gairdner Hospital, Perth, Australia; Quinn, J. Quinn, Chicago Infectious Disease Research Institute, Chicago, IL.