Fig. 10.

Recruitment of endogenous SP1-C/EBPβ-ERα onto hPIII promoter by ChIP analysis. The upper graphs show CHIP performed with chromatin from MCF-7A2 cells transiently transfected with the coding region of siSP1 (II), siC/EBPβ (III), or siERα (IV) (30 nM) followed by treatment with or without E2 (100 nM). Scramble siRNA (I) was used as the negative control. The promoter region of hPIII containing SP1-C/EBPβ (−497/−321) was analyzed by PCR following immunoprecipitation (IP) with the antibody indicated. IgG was used as a negative control. The lower panels show the experimental procedure and a diagram of protein complex interaction onto the hPIII promoter.