Fig. 2.

Identification of domain of ERα responsible for the interaction with SP1 or C/EBPβ. (A) A schematic presentation of the V5 epitope-tagged wild-type (WT) and deletion forms (mE1 to mE7) of human ERα. (B) In vitro-translated proteins from various forms of ERα were used in a pulldown assay. GST-SP1 (C) or GST-C/EBPβ (D) purified by GST-affinity resin was incubated with the in vitro-translated wild-type or deleted form of ERα-V5 in the presence or absence of 17β-estradiol (E2; 100 nM). The inputs and interacting forms of ERα-V5 were detected by Western blot analysis using V5 antibody. Results are representative of three independent experiments.