Fig. 4.

Identification of domains of C/EBPβ responsible for interaction with ERα or SP1. (A) A schematic presentation of V5 epitope-tagged wild-type (WT) or deletion forms (mC1 to mC3) of human C/EBPβ. GST-ERα (B) or GST-SP1 (C) was incubated with each form of in vitro-translated C/EBPβ-V5 in the presence or absence of 17β-estradiol (E2; 100 nM). The inputs and interacting forms of C/EBPβ-V5 were detected by Western blot analysis using V5 antibody. Results are representative of three independent experiments.