Skip to main content
. 2011 Aug;31(16):3378–3395. doi: 10.1128/MCB.05087-11

Fig. 1.

Fig. 1.

The Ran protein gradient in interphase cells depends on the nuclear lamina and is correlated with markers of heterochromatin. (A) Ran distribution in primary fibroblasts from a control individual (Normal 8469) and three progeria patients (HGPS 1972, HGPS 1498, and HGPS 3199). DAPI, 4′,6-diamidino-2-phenylindole. (B) Histograms (bin size = 0.5) of Ran N/C ratios from control (Normal 8469) (black bars; n = 293) and HGPS patient (HGPS 1972, HGPS 1498, and HGPS 3199) (red lines; n = 264, 53, and 207, respectively) cells. (C) Ran N/C values plotted as a function of nuclear H3K9me3 levels (Spearman's rank correlation coefficient [ρ] values of 0.81 for Normal 8469, 0.74 for HGPS 1972, 0.81 for HGPS 1498, and 0.29 for HGPS 3199). (D) Nuclear Ran values plotted as a function of nuclear HP1γ levels (ρ values of 0.81 for Normal 8469, 0.74 for HGPS 1972, 0.81 for HGPS 1498, and 0.29 for HGPS 3199; n = 64, 50, 40, and 108, respectively). AU, arbitrary units. (E) Immunoblotting of primary fibroblasts. (F) Colocalization of transiently transfected HA-tagged WT lamin A and HA-progerin (red) with endogenous Ran (green) in HeLa cells. Scale bars, 20 μm.