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. 2011 Aug;31(16):3378–3395. doi: 10.1128/MCB.05087-11

Fig. 13.

Fig. 13.

SUMOylation regulates RCC1-chromatin interactions. (A) Localizations of mCherry-SUMO2 (red) and RCC1-GFP (green) in HeLa cells cotransfected HA-WT lamin A, HA-progerin, or the FLAG-SENP CD. (B) Nuclear mobility of RCC1-GFP measured by FRAP of cells expressing WT lamin A (n = 31), progerin (n = 24), or the SENP CD (n = 29). Error bars represent standard errors of the means (SEM). (C) SUMOylation-induced dissociation of RCC1 from chromatin. HeLa cell chromatin prebound with His-Ran was incubated with the combinations of recombinant factors indicated. Following a centrifugation step, RCC1 and Ran in supernatant and pellet fractions were analyzed by immunoblotting. Histones in the pellet fraction were visualized by Ponceau S staining. (D) The T24N form of Ran inhibits the SUMOylation-induced dissociation of RCC1 from chromatin. HeLa cell chromatin prebound with GST-Ran (WT and T24N) was treated as described above (C).