Fig. 4.

ScSir4 is not associated with promoters of sporulation genes. (A) The association of ScHst1, ScSir2, and derivatives with the ScDTR1 and ScPES4 promoters was examined by chromatin IP (ChIP). HA-tagged proteins were precipitated from an Scsir2Δ Schst1Δ ScRFM1–myc mutant yeast strain (LRY2507) transformed with the empty vector or plasmids containing the constructs shown in Fig. 3A. Associated DNA was analyzed by quantitative PCR, and enrichment is expressed relative to the ScATS1 locus, which is not associated with ScSir2 or ScHst1. (B) The association of ScSir4 with the ScDTR1 and ScPES4 promoters and the HML–E silencer was examined by ChIP as in panel A.