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. 2011 Aug;31(16):3485–3496. doi: 10.1128/MCB.05599-11

Fig. 4.

Fig. 4.

The region of low histone occupancy induced by 601 differs from NDRs at promoters. (a) Growth defect of the sth1-td strain (DBY1229) at 37°C with 2 μg/ml doxycycline. WT, wild type. (b, c) Histone H3 occupancy at the control SCR1 (29) locus and in GAL1-YLR454W-601fw+1242 before and after Sth1 depletion for 2 h at 37°C with 2 μg/ml doxycycline. Note that Sth1 inactivation increases histone occupancy at SCR1 as expected but not around the 601 element in GAL1-YLR454W. Mean values normalized to TELVIR (TEL) with standard deviations are shown. Dox, doxycycline. (d) Histone Htz1 ChIP using tiled Q-PCR amplicons in DBY1191 GAL1-YLR454W-601fw+1242 and in the control strain without a 601 insertion relative to the BPH1 promoter, which is a positive control for Htz1 (31). Low signals for 601 amplicons (underlined) in the control strain are background. Mean values from n PCRs from at least 2 IPs with standard deviations are shown.