Fig. 6.

Effects of transcriptional history on nucleosome occupancy at 601. Shown are relative pol II (a), histone H3 (b), and H2B (c) ChIP signals at the GAL1-YLR454W-601fw+1242 gene in cells grown in Lac and during a cycle of galactose activation (30 min, +Gal-1), glucose repression (120 min, +Glu), and galactose reactivation (30 min, +Gal-2). Note that histone occupancy at 601 increased on the first activation (white versus green bars in panel b and white versus red bars in panel c) and decreased on the second activation (gray versus blue bars in panels b and c). Means and standard deviations for n PCRs are normalized to the input in panel a and to TELVIR in panels b and c.