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. 2011 Aug;31(16):3485–3496. doi: 10.1128/MCB.05599-11

Fig. 7.

Fig. 7.

Spt16/FACT-dependent histone deposition at 601. (a, b) Anti-H3 ChIP of the GAL1-YLR454W-601rev+1242 and GAL1-YLR454W-601fw+5550 genes in Lac and induced with galactose (Gal) for 30 min. Note that, as in Fig. 6b, H3 occupancy decreased at most locations on Gal induction, but in contrast, it slightly increased at 601. (c) Anti-H3 ChIP of the GAL1-YLR454W-601fw+1242 gene (DBY1109 spt16ts) in Lac or galactose (Gal) at 25°C or 34°C. Note histone deposition at 601 when the gene was activated at 25°C and eviction at 34°C. Means and standard deviations for n PCRs are normalized to TELVIR.