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. 2011 Aug;31(16):3485–3496. doi: 10.1128/MCB.05599-11

Fig. 8.

Fig. 8.

Transcriptional history determines nucleosome occupancy flanking 601. (a) Experimental design and map of Q-PCR primer pairs used for MNase sensitivity mapping. Position numbers represent the middle of each amplicon relative to the ATG. Primer pairs overlapping 601 are in red. MNase sensitivity of chromatin at GAL1-YLR454W (b) in DBY446 and in GAL1-YLR454W-601fw+1242 (c) and GAL1-YLR454W-601rv+1242 (d) in cells grown in Lac or repressed with glucose (Glu) after galactose activation. Dashed lines represent the moving average of the values at each amplicon. Note the MNase sensitivity across the 601 dyad at position 1395 (red arrow in panel c and blue arrow in panel d). Amplicons in inverted 601 are numbered relative to the AIG. Mean values from 2 IPs and 4 PCRs per IP with standard deviations are normalized to the signals at the PHO5 −1 nucleosome.