Fig. 2.

Deletion of the extreme amino terminus of Swi1 abolishes aggregation in [SWI⁺] cells. (A) Microscopy images (×100) of [swi⁻] and [SWI⁺] cells expressing Swi1N amino-terminal truncation mutants fused to YFP. Ten to 20 individual transformants were imaged for each truncation; representative images are shown. The numbers indicate the average percentages of [SWI⁺] cells with aggregates (agg). (B) Western blot of the Swi1N amino-terminal-truncation YFP fusions. A polyclonal anti-GFP antibody was used to detect the fusion proteins.