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. 2011 Aug;31(16):3436–3444. doi: 10.1128/MCB.05338-11

Fig. 6.

Fig. 6.

Swi11-37 can transmit [SWI+] to endogenous Swi1. (A) Schematic of experimental design. A swi1Δ [SWI+]/p415TEF-SWI11-37GFP strain (donor) was cytoduced to a [swi−] kar1-1/p416TEF-SWI1-mCherry strain (recipient). Cytoductants were selected for on media lacking uracil and leucine. Authentic cytoductants were selected based on the presence of the recipient cell nuclear marker (Met+) and the absence of the donor cell nuclear marker (Lys+). (B) Microscopy images (×100) of cytoductant, as well as [swi−] and [SWI+] control strains containing p416TEF-SWI1mCherry. Seven cytoductants were imaged; a representative image of a cytoductant exhibiting Swi1-mCherry aggregation is shown. (C) Log-phase cells were serially diluted 1:10 and spotted to YPD and raffinose media. Seven cytoductants were tested; representative results are shown.