Table 1.
Primers used in this study
| Primer name | Primer sequencea | Resulting plasmid |
|---|---|---|
| Swi1N rev | 5′-TGGATCCCGGGCAGAGAACA-3′ | Amino-terminal truncations |
| Swi1N fwd | 5′-ATCTAGAACTAGTATGGTTTCTTTAATTT-3′ | Carboxy-terminal truncations |
| 55 fwd | 5′-TACTACTAGTATGAACAATACTAATAACAACAATACGAATACC-3′ | p416TEF-SWI155-327YFP |
| 100 fwd | 5′-TACTACTAGTATGAATAACAGTAACACGGTAGCCTCC-3′ | p416TEF-SWI1100-327YFP |
| 156 fwd | 5′-TACTACTAGTATGAACAGCCTGTCTCCTCAGGC-3′ | p416TEF-SWI1156-327YFP |
| 206 fwd | 5′-TACTACTAGTATGATCACCAATGTTCAATCCATCAG-3′ | p416TEF-SWI1206-327YFP |
| 250 fwd | 5′-TACTACTAGTATGTCTAACCAGCTTATTTCTAATTACGC-3′ | p416TEF-SWI1250-327YFP |
| 225 rev | 5′-GGTGGATCCTTTGTGTTCGG-3′ | p416TEF-SWI11-225YFP |
| 176 rev | 5′-GGTGGATCCAAATTGGAAGAATC-3′ | p416TEF-SWI11-176YFP |
| 134 rev | 5′-GGTGGATCCTTAGCAGCTTTTC-3′ | p416TEF-SWI11-134YFP |
| 74 rev | 5′-GGTGGATCCTGAAAATCGTCTAC-3′ | p416TEF-SWI11-74YFP |
| 65 rev | 5′-GGTGGATCCTTGGTATTCGTATTGTTGTTATTAGTATTG-3′ | p416TEF-SWI11-65YFP |
| 46 rev | 5′-GTTGGATCCCTATTGTTGTTATTGGTATTATTAGCAGG-3′ | p416TEF-SWI11-46YFP |
| 41 rev | 5′-GTTGGATCCGTATTATTAGCAGGATTATTATTATT-3′ | p416TEF-SWI11-41YFP |
| 37 rev | 5′-GTTGGATCCGGATTATTATTATTATTAGTATTATTATT-3′ | p416TEF-SWI11-37YFP |
| 31 rev | 5′-GTTGGATCCGTATTATTATTATTAGTATTATTGTTATT-3′ | p416TEF-SWI11-31YFP |
| GFP fwd | 5′-TATGGATCCATCTTTAATTAACAGTAAAGGAGAAGAAC-3′ | p416TEF-SWI11-37GFP |
| GFP rev | 5′-ATACTCGAGCTATTTGTATAGTTCATCCATGCCATG-3′ | p416TEF-SWI11-37GFP |
| mCherry fwd | 5′-TATCTCGAGGTGAGCAAGGGCGAGGAG-3′ | p416TEF-SWI1mCherry |
| mCherry rev | 5′-TATCTCGAGCTACTTGTACAGCTCGTCCATG-3′ | p416TEF-SWI1mCherry |
a
The underlined nucleotides indicate restriction sites used for cloning.