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LOX-PP reduces the nuclear levels of the PIC isoform in lung cancer cells. H1299 cells were cotransfected with GFP-PIC and a V5-tagged LOX-PP construct or EV DNA as a control. For LOX-PP staining, a V5 antibody and anti-mouse Alexa Fluor 555-IgG secondary antibody (red) were used. Nuclei were labeled with DAPI (blue). Fluorescence microscopy was performed using a Nikon Eclipse E400 microscope and a 100× objective. Individual and merged images are shown. Bars = 60 μm.
