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Replication timing of cells transfected with the LCR–Pro–EGFP–Rep-PAG2 cassette before zebularine treatment (A) and after zebularine treatment (B). We quantified newly replicated transgene (Rep-P) DNA and host sequences (mBG and mAmylase) at different cell cycle phases by real-time PCR. mBG, mouse β-globin locus, an early replicating region in mouse MEL cells; mAmylase, mouse amylase gene, a late replicating region; Rep-P, the Rep-PAG2 region of the inserted transgene cassette.
