Table 1.
List of primers and probes for real-time PCR
| Primer or probea | Sequence |
|---|---|
| bG59.8 | |
| Fwd primer | TGGAAAAGCAACCCCTGC |
| Rev primer | AACTATGGATCCTTCTCTTGTGTTGG |
| Probeb | GCTGCAGATACCATCATCCTGGCTTCAA |
| LB2 | |
| Fwd primer | TGGGACCCTGCCCTTTTT |
| Rev primer | CGTGACGAAGAGTCAGCT |
| Probeb | TTCTAGTGAGCCTCCGAC |
| Rep-I | |
| Fwd primer | GGTGAAGGCTCATGGCAAGA |
| Rev primer | AAAGGTGCCCTTGAGGTTGTC |
| Probeb | CCTTTAGTGATGGCCTGGCTCACCTG |
| hBG | |
| Fwd primer | TGAGGGTTTGAAGTCCAACTCC |
| Rev primer | GGTCTAAGTGATGACAGCCGTACC |
| Probeb | AAGCCAGTGCCAGAAGAGCCAAGGA |
| mAmy | |
| Fwd primer | TCATATTCTAATCAAGACTAGTGACTTTAGAGC |
| Rev primer | TGCCACAACTACCAATCCTTTT |
| Probe | CAACTTCATTTCACACATGACTTTGCTGAGAAA |
| GFP | |
| Fwd primer | AGCAAAGACCCCAACGAGAA |
| Rev primer | GGCGGCGGTCACGAA |
| Probeb | CGCGATCACATGGTCCTGCTGG |
| mBG | |
| Fwd primer | CCAGCCTCAGTGAGCTCCA |
| Rev primer | CCCATCAGACTCACCCTGAAG |
| Probeb | TGTGACAAGCTGCATGTGGATCCTGA |
| mCh15 | |
| Fwd primer | TCCGTCCCCTTCTCCTCC |
| Rev primer | TTCAGGTTCCATTGCCACG |
| Probeb | CACCATTCACACAGCCCACGAGCA |
| AG | |
| Fwd primer | CAACTCCTAAGCCAGTGCCAGAAG |
| Rev primer | TGCCCTGACTTTTATGCCCAGC |
| Probeb | TCATCACTTAGACCTCACCCTGT |
| HS2 | |
| Fwd primer | TGTGTAACCTTCTAAGCAAACCTTCTG |
| Rev primer | GCCATCTGCCCTGTAAGCATC |
| Probeb | GTTCTCAGCCTAGAGTGATGAC |
a
The forward (Fwd) and reverse (Rev) primers and probe are shown for Rep-P 5′-end sequence (bG59.8), lamin B2 replicator (LB2), replicator Rep-I in the β-globin locus, human β-globin promoter (hBG), mouse amylase gene (mAmy), enhanced green fluorescent protein (EGFP) gene (GFP), mouse β-globin locus (mBG), murine chromosome 15 (mCh15), the AG region of Rep-P, and hypersensitive site 2 (HS2).
b
These probes were modified with 6-carboxyfluorescein (FAM) at the 5′ end and with 6-carboxytetramethylrhodamine (TAMRA) at the 3′ end.