Fig. 3.

Alignments of Pfr364 and Pvr47 family members with PCR primers. (A) Pfr364 with primers. Arrows represent locations of PCR primers in context of the full alignment. The full alignment is 1,538 positions in length; here a partial alignment is shown. Vertical black lines indicate where the sequence alignment has been truncated to enable viewing of all 4 primer locations. The alignment shows two subfamilies within Pfr364. We have designated the upper 22 sequences subfamily 1 and the lower 19 sequences subfamily 2. Forward and reverse primer pairs used for multiplex and conventional PCR are, respectively, the last two sequence pairs in the alignment. (B) Pvr47 with primers. Arrows represent locations of PCR primers in context of the full alignment. The full alignment is 1,070 positions; here only positions 433 to 776 are shown. Vertical lines indicate where the sequence alignment has been truncated for easier viewing. Forward and reverse primers are, respectively, the last two sequences in the alignment.