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Limits of detection for conventional PCR assays. Primers to novel targets P. falciparum Pfr364 (A) and P. vivax Pvr47 (B) were used to amplify parasite DNA of the appropriate species. DNA was quantified, and 10-fold serial dilutions from 10,000 parasites/μl (lane 1) to 0.01 parasites/μl (lane 7) were used to determine the limit of detection. A 100-bp standard ladder (L) and a no-template control (NTC) were included.
