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Multiplex PCR. The multiplex method clearly identified mock mixed P. falciparum and P. vivax infections (lane Pf/Pv). Single-species infections (lanes Pf and Pv) were also detected. The P. falciparum band appears at 220 bp and the P. vivax band at 333 bp. A 100-bp standard ladder (L) and a no-template control (NTC) were used.
