Table 1.
New target primer sequencesa
| Primer | Sequence |
|
|---|---|---|
| P. falciparum Pfr364 | P. vivax Pvr47 | |
| Forward | 5′-CCATTTTACTCGCAATAACGCTGCAT | 5′-CTGATTTTCCGCGTAACAATG |
| Reverse | 5′-CTGAGTCGAATGAACTAGTCGCTAC | 5′-CAAATGTAGCATAAAAATCYAAG |
| Alt-Forward | 5′-CCGGAAATTCGGGTTTTAGAC | |
| Alt-Reverse | 5′-GCTTTGAAGTGCATGTGAATTGTGCAC | |
a
Primer sequences designed to targets Pfr364 and Pvr47. The alternate (Alt) primer pair for P. falciparum was used in multiplex reactions only. The P. vivax primer set was the same for both single-species and multiplex PCRs.