Table 5.
Sequencing results of nasopharyngeal samples from the pneumonia group positive with the new pan-Chlamydiales PCR
| Patient no. | Sexa | Age (yr) | Signs and symptomsb | Other etiology | Underlying condition(s) | % 16S rRNA gene homology with most similar GenBank sequence (corresponding family)c |
|---|---|---|---|---|---|---|
| GE10160 | F | 3.7 | 39.0°C, cough, thoracic pain, RDS | coeliakie | 100% Chlamydia pneumoniae LPCoLN (Ch) | |
| GE10097 | F | 2.7 | 40.6°C, cough, RDS | 100% Chlamydia pneumoniae LPCoLN (Ch) | ||
| VS30014 | M | 12.4 | 38.9°C, cough | 100% Chlamydia pneumoniae LPCoLN (Ch) | ||
| VS30030 | F | 12.2 | 39.5°C, cough | 100% Chlamydia trachomatis D-LC (Ch) | ||
| GE10098 | F | 8.0 | 38.0°C, cough | 100% Chlamydia pneumoniae CWL029 (Ch) | ||
| GE10014 | M | 7.6 | 39.5°C, cough | M. pneumoniae | 94% uncultured Neochlamydia sp. strain LTUNC09656 (P) | |
| GE10159 | M | 3.7 | 40.0°C, cough | 100% Chlamydia pneumoniae LPCoLN (Ch) | ||
| GE10169 | M | 5.6 | 40.0°C, cough, thoracic pain, tachypnea | Bronchodysplasia, premature birth (28 weeks) | 97% “Candidatus Rhabdochlamydia porcellionis” (R); 91% Chlamydiales bacterium cvE38 (S) | |
| GE10179 | F | 3.6 | Dyspnea | S. pneumoniae H1N1 virus | Sequencing failed | |
| HE20032 | M | 1.5 | 41.6°C, cough, RDS | 94% uncultured “Candidatus Protochlamydia sp.” clone CN823 (P) | ||
| VS30003 | M | 5.8 | 39.5°C, cough, RDS | 95% uncultured Chlamydiales bacterium clone P-5 (P) | ||
| GE10027 | M | 9.8 | 38.1°C, cough, RDS | M. pneumoniae | asthma | 92% uncultured soil bacterium clone 530-2 (Cr) |
| GE10036 | M | 1.6 | 38.0°C, cough, RDS, tachypnea | S. pneumoniae | 95% uncultured bacterium clone F5K2Q4C04JDDHX (P) | |
| GE10047 | M | 2.6 | 39.5°C, cough, RDS, tachypnea | 92% Criblamydia sequanensis (Cr) | ||
| GE10072 | F | 4.8 | 39.5°C | HMPV A | Sequencing failed | |
| GE10147 | M | 13.8 | 38.2°C, cough, RDS | S. pneumoniae | 95% uncultured bacterium clone FW1013-189 (P) | |
| GE10193 | F | 5.1 | 38.9°C, cough, tachypnea | 92% Chlamydiales bacterium cvE38 (S) | ||
| HE20008 | F | 3.3 | 38.3°C, cough, tachypnea, RDS | HUK1 | 93% Estrella lausannensis strain CRIB 30 (Cr) | |
| HE20028 | F | 1.1 | 39.5°C, cough, RDS, tachypnea | HRSV A | 94% uncultured Chlamydiales bacterium clone P-5 (P) | |
| HE20036 | M | 1.4 | 40.0°C, RDS | 96% Chlamydiales bacterium cvE21 (E6) | ||
| HE20074 | M | 4.5 | 38.1°C, cough, RDS | 94% Criblamydia sequanensis (Cr) | ||
| VS30007 | M | 6.6 | 38.7°C, cough, RDS | 95% “Candidatus Metachlamydia lacustris” strain CHSL (P) | ||
| VS30013 | M | 6.4 | 38.5°C, cough, tachypnea | 94% uncultured Chlamydiales bacterium clone P-9 (P) | ||
| VS30044 | M | 3.8 | 40.4°C, cough | 95% uncultured Chlamydiales bacterium clone P-7 (P) | ||
| VS30055 | F | 4.1 | 38.5°C, cough, tachypnea | 92% “Candidatus Metachlamydia lacustris” strain CHSL (P) |
a
F, female; M, male.
b
RDS, respiratory distress syndrome.
c
Ch, Chlamydiaceae; P, Parachlamydiaceae; R, Rhabdochlamydiaceae; S, Simkaniaceae; Cr, Criblamydiaceae; E6, novel E6 lineage.