Table 2.
Assay limit of detection (LoD) with 2009 A (H1N1) pdm and classical swine influenza viruses
| Influenza virus and infectious titer | rRT-PCR resultsc |
LoD | ||
|---|---|---|---|---|
| InfA | swInfA | swH1 | ||
| A/California/07/2009a | ||||
| 104.4 | +/+/+ | +/+/+ | +/+/+ | |
| 103.4 | +/+/+ | +/+/+ | +/+/+ | |
| 102.4 | +/+/+ | +/+/+ | +/+/+ | |
| 101.4 | +/+/+ | +/+/+ | +/+/+ | 101.4 |
| 100.4 | −/−/− | −/−/− | −/−/− | |
| 10−0.4 | −/−/− | −/−/− | −/+/− | |
| A/California/04/2009b | ||||
| 104.6 | +/+/+ | +/+/+ | +/+/+ | |
| 103.6 | +/+/+ | +/+/+ | +/+/+ | |
| 102.6 | +/+/+ | +/+/+ | +/+/+ | |
| 101.6 | +/+/+ | +/+/+ | +/+/+ | 101.6 |
| 100.6 | +/+/− | +/+/+ | +/+/+ | |
| 10−0.6 | −/−/− | −/−/− | −/−/− | |
| 10−1.6 | −/−/− | −/−/− | −/−/− | |
| A/Maryland/12/1991a | ||||
| 105.0 | +/+/+ | +/+/+ | +/+/+ | |
| 104.0 | +/+/+ | +/+/+ | +/+/+ | |
| 103.0 | +/+/+ | +/+/+ | +/+/+ | |
| 102.0 | +/+/+ | +/+/+ | +/+/+ | |
| 101.0 | +/+/+ | +/+/+ | +/+/+ | 101.0 |
| 100.0 | −/−/− | +/+/− | +/+/− | |
| 10−1.0 | −/−/− | −/−/− | −/−/− | |
a
Data represent EID50/ml values.
b
Data represent TCID50/ml values.
c
+, positive rRT-PCR result; −, negative rRT-PCR result.