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Assay limit of detection determined using quantified RNAa
| No. of RNA copies |
CT values for duplicate assays |
||
|---|---|---|---|
| InfA | swInfA | swH1 | |
| 500 | 31.30/31.71 | 33.17/33.56 | 33.14/33.45 |
| 50 | 34.54/34.49 | 37.44/37.46 | 37.05/37.12 |
| 5 | 37.88/38.04 | 39.54/40.98 | 39.06/40.32 |
| 1 | −/− | −/− | −/− |
| 0.1 | −/− | −/− | −/− |
The RNA material [Armored RNA Quant Flu A (H1N1) 2009; received from AsuraGen, Inc.] included HA, NP, and M gene region sequences derived from 2009 A (H1N1) pdm influenza virus. −, negative rRT-PCR results.
