Fig. 2.

Evaluation of a WT-selective PCR blocker by SYBR green real-time PCR. Approximately 100 femtograms (∼3 × 10⁴ copies) (A) or 1 nanogram (∼3 × 10⁸ copies) (B) of plasmids carrying either the WT sequence (lines 3, 4, 7, and 8) or the A1762T/G1764A mutation (lines 1, 2, 5, and 6) was amplified in the presence (lines 2, 4, 6, and 8) or absence (lines 1, 3, 5, and 7) of the PCR blocker for 45 cycles of 95°C for 10 s, 57°C for 10 s, and 65°C for 5 s using the forward primer F1 and the reverse primer R1.