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. 2011 Jul;49(7):2440–2448. doi: 10.1128/JCM.02472-10

Fig. 5.

Fig. 5.

Effect of primer-blocker-probe partial overlap (PBPPO) on the performance of the PCR. (A) PBPPO prevents blocker leakage. Line 1, WT, no blocker; line 2, WT/1753C/1757A, no blocker; line 3, WT, with blocker; line 4, WT/1753C/1757A, with blocker. (B) PBPPO simplifies melting curve patterns. Line 1, WT template amplified with F1 and R1; line 2, A1762T/G1764A template amplified with F1 and R1; line 3, WT/1753C/1757A template amplified with F2 and R1; line 4, WT/1753C/1757A template amplified with F1 and R1; line 5, A1762T/G1764A/1753C/1757A template amplified with F2 and R1; line 6, A1762T/G1764A/1753C/1757A template amplified with F1 and R1. (C and D) The amplification efficiencies of A1762T/G1764A (line 1) and A1762T/G1764A/1753C/1757A (line 2) plasmids were examined in a SYBR green PCR using primers F1/R1 (C) or F2/R1 (D).